STIMULATION BY T CELL INDEPENDENT ANTIGENS CAN RELIEVE THE ARREST OF DIFFERENTIATION OF IMMATURE AUTO-REACTIVE B CELLS IN THE BONE MARROW

摘要: The pair of microH-chain and kappa L-chain transgenes encoding the Sp6 TNP/DNA-specific IgM was bred onto rearrangement-deficient genetic background RAG-2T mice, expression-deficient iE T mice. Bone marrow transgenic mice contained normal numbers B220(CD45R)+c-kit+ pro/preB-I-like cells B220(CD45R)+TAC+ preB-II-like cells. Most strikingly, immature sIgM+ B in bone were at least five-fold lower than normal, while mature almost undetectable as well spleen. Hence, cell development these appears to be arrested transition from preB-II contents spleen different precursors, compartments Sp6iE found similar those except that all sIg+ expressed lambda L-chains, which 40% coexpressed L-chain. It indicates repertoire rearrangements L-chains it suffices relieve arrest differentiation seen Sp6RAG-2T cell-independent antigen TNP-Ficoll elicited within 5 days a response develop IgM-secreting fill serum pool with 100 micrograms/ml, i.e. levels interfere differentiation. Two scenarios for this its relief by T-independent are discussed.