C-Jun NH(2)-terminal kinase mediates proliferation and tumor growth of human prostate carcinoma.

摘要: Purpose: C-Jun NH2-terminal kinase (JNK) has been implicated in numerous functions including stress responses, apoptosis,and transformation. The role transformation is based largely on studies of isolated cell types with little indication whether JNK plays a general specific human tumor type or this occurs vivo . Experimental Design: We examined 9 prostate carcinoma lines vitro and representative line . Results: For all the proliferation highly correlated serum-supported activity (rPearson = 0.91; P 0.004), whereas no relationship was observed for 10 breast cancer −0.32). Treatment characterized antisense oligonucleotides complementary to sequences common either JNK1 JNK2 family isoforms showed that, inhibited growth by maximum 57%, up 80%. Sense scrambled control had effect (average 3.7 ± 1.5%). Moreover, systemic treatment mice bearing established xenografts PC3 cells led inhibition 57% ( < 0.002) 80% 0.001), respectively. difference significant 0.012). Combined increase frequency regression 0.022). Conclusion: These results indicate that required , additionally dominant role. pathway novel target carcinoma.