Maintenance of EGFR and EGFRvIII expressions in an in vivo and in vitro model of human glioblastoma multiforme.

摘要: Glioblastoma multiforme (GBM) is the most common, and aggressive primary brain tumor among adults. A vast majority of tumors express high levels epidermal growth factor receptor (EGFR) as a consequence gene amplification. Furthermore, amplification often associated with mutation EGFR, constitutive activated deletion variant EGFRvIII common EGFR found in GBM. Activated signaling, through overexpression and/or mutation, involved increased tumorigenic potential. As such, an attractive target for GBM therapy. However, clinical studies inhibitors have shown inconsistent results, further knowledge regarding role needed. For this, appropriate vivo/in vitro model required. Here, we report establishment experimental which expressions are maintained both xenograft growing subcutaneously on mice cell cultures established stem conditions. With this it will be possible to study EGFRvIII, response targeted therapy,