The heritable changes in metabolic profiles of plants regenerated in different types of in vitro culture
作者： Anita Wiśniewska , Zhimin Yin , Stefan Malepszy , Marcin Filipecki
DOI: 10.1007/S11240-005-2585-8
关键词:
摘要: In this work we show how three types of cucumber in vitro cultures – leaf callus culture, cytokinin dependent cell suspension and liquid culture meristematic clumps influence the metabolite profiles plants first generative progeny. Based on study conclude that there exists a specific inheritable metabolic fingerprint reflecting history previous generations, probably related to stress factors accompanying passage through different culture. The generated highest heritable differences content was most distinctly separated cluster PCA analysis. smallest number variable metabolites characterizes regenerated from whereas induced slightly more changes. Changes by these two were not as pronounced case However after well control diagram. changes over 2-fold increases cystin galactose-6-P decreases aspartate, myo-inositol, hydroxylamine, phosphate putrescine. These concerned plants, which one generation possible nature observed is discussed.
springer.com
sci-hub.se 参考文章(17)
S Malepszy, H O Mackiewicz, Obtaining and characterization of tetraploid forms in cucumber - Cucumis sativus L.var. sativus and var. hardwickii Alef. Folia Horticulturae. ,vol. 08, ,(1996)
Maciej Ładyżyński, Wojciech Burza, Stefan Malepszy, Relationship between somaclonal variation and type of culture in cucumber Euphytica. ,vol. 125, pp. 349- 356 ,(2002) , 10.1023/A:1016017825907
W. Plader, S. Malepszy, W. Burza, Z. Rusinowski, The relationship between the regeneration system and genetic variability in the cucumber (Cucumis sativus L.) Euphytica. ,vol. 103, pp. 9- 15 ,(1998) , 10.1023/A:1018359726626
Oliver Fiehn, Joachim Kopka, Peter Dörmann, Thomas Altmann, Richard N. Trethewey, Lothar Willmitzer, Metabolite profiling for plant functional genomics Nature Biotechnology. ,vol. 18, pp. 1157- 1161 ,(2000) , 10.1038/81137
Shirley Anderson, Aidan C. Lewis-Smith, Steven M. Smith, Methylation of ribosomal RNA genes in Petunia hybrida plants, callus cultures and regenerated shoots Plant Cell Reports. ,vol. 8, pp. 554- 557 ,(1990) , 10.1007/BF00820208
Olga Kovalchuk, Paula Burke, Andrey Arkhipov, Nikolaj Kuchma, S.Jill James, Igor Kovalchuk, Igor Pogribny, Genome hypermethylation in Pinus silvestris of Chernobyl--a mechanism for radiation adaptation? Mutation Research. ,vol. 529, pp. 13- 20 ,(2003) , 10.1016/S0027-5107(03)00103-9
R. L. Phillips, S. M. Kaeppler, P. Olhoft, Genetic instability of plant tissue cultures: breakdown of normal controls Proceedings of the National Academy of Sciences of the United States of America. ,vol. 91, pp. 5222- 5226 ,(1994) , 10.1073/PNAS.91.12.5222
W. Burza, S. Malepszy, Direct plant regeneration from leaf explants in cucumber (Cucumis sativus L.) is free of stable genetic variation Plant Breeding. ,vol. 114, pp. 341- 345 ,(1995) , 10.1111/J.1439-0523.1995.TB01246.X
P. J. Larkin, W. R. Scowcroft, Somaclonal variation — a novel source of variability from cell cultures for plant improvement Theoretical and Applied Genetics. ,vol. 60, pp. 197- 214 ,(1981) , 10.1007/BF02342540
S. M. Kaeppler, R. L. Phillips, Tissue culture-induced DNA methylation variation in maize Proceedings of the National Academy of Sciences of the United States of America. ,vol. 90, pp. 8773- 8776 ,(1993) , 10.1073/PNAS.90.19.8773