p53 mutations are associated with 17p allelic loss in grade II and grade III astrocytoma.

摘要: Loss of genetic material on the short arm chromosome 17 is observed in approximately 40% human astrocytomas (WHO grades II and III) 30% cases glioblastoma multiforme grade IV). Previous studies have shown that p53 gene, located 17, frequently mutated these glioblastomas. To explore whether lower-grade are also associated with corresponding mutations we investigated a series 22 WHO III both for loss heterozygosity 17p mutations. Mutations conserved regions gene were identified by single strand conformation polymorphism analysis exons 5, 6, 7, 8 verified direct DNA sequencing polymerase chain reaction products. 3 4 14 astrocytomas. In all tumors, allelic was restriction fragment length analysis. One-half (4 8) (7 14) exhibited 17p. exclusively tumors Our results show not restricted to may be important tumorigenesis These findings consistent recessive mechanism action astrocytoma tumorigenesis.