Development of an HIV-1 reference panel of subtype B envelope clones isolated from the plasma of recently infected individuals.

摘要: We have developed an HIV-1 reference panel of 20 subtype B envelope clones isolated from the plasma recently infected individuals. It is widely accepted that a prophylactic vaccine against requires development novel immunogens are capable eliciting broadly protective neutralizing antibody responses. Historically, patient serum has been screened for such antibodies by assaying laboratory strains, but these viruses typically increased neutralization sensitivity compared with primary isolates. To create more standardized and relevant assay system evaluation, we envelopes derived individuals documented acute/early infection occurring between 2000 2004. The this vary in mode transmission, coreceptor tropism, fusogenicity, overall to neutralization. These sequences represent broad spectrum genetic diversity average pairwise distance 12% range 10% 19%. This well-characterized should be valuable resource optimizing standardizing assessment improving design.