Regulation of epidermal growth factor receptor in NIH3T3/HER14 cells by antireceptor monoclonal antibodies.
作者： Z Fan , J Mendelsohn , H Masui , R Kumar
DOI: 10.1016/S0021-9258(19)36895-4
关键词:
摘要: The mechanism(s) by which monoclonal antibodies (mAbs) against the epidermal growth factor (EGF) receptor regulate function have been investigated with NIH3T3/HER14 fibroblasts expressing human EGF receptors. Bivalent 225 mAb or monovalent Fab' inhibited transforming (TGF)-alpha-induced tyrosine phosphorylation and cell proliferation. Culture of HER14 cells did not activate kinase when assayed after lysis in SDS sample buffer. However, were cultured bivalent mAb, but Fab', subsequently lysed further incubated Triton X-100 buffer containing proteinase phosphatase inhibitors, was observed. Phosphorylation confined to residues addition genistein lysis, indicating that it due activation protein kinase. activity unphysiologic, contrast TGF-alpha, occurred only delayed kinetics; serine threonine occur; down-regulation receptors slower. Selective mAb-mediated on sufficient a SH2 group-bearing substrate, phospholipase C-gamma, serine/threonine is required for activity. These studies provide novel insights into capacity modulate function.
sci-hub.st 参考文章(42)
B Ek, C H Heldin, Characterization of a tyrosine-specific kinase activity in human fibroblast membranes stimulated by platelet-derived growth factor. Journal of Biological Chemistry. ,vol. 257, pp. 10486- 10492 ,(1982) , 10.1016/S0021-9258(18)34044-4
M Torti, K.B. Marti, D Altschuler, K Yamamoto, E.G. Lapetina, Erythropoietin induces p21ras activation and p120GAP tyrosine phosphorylation in human erythroleukemia cells. Journal of Biological Chemistry. ,vol. 267, pp. 8293- 8298 ,(1992) , 10.1016/S0021-9258(18)42441-6
J A Fernandez-Pol, Epidermal growth factor receptor of A431 cells. Characterization of a monoclonal anti-receptor antibody noncompetitive agonist of epidermal growth factor action. Journal of Biological Chemistry. ,vol. 260, pp. 5003- 5011 ,(1985) , 10.1016/S0021-9258(18)89171-2
M Spaargaren, L H Defize, J Boonstra, S W de Laat, Antibody-induced dimerization activates the epidermal growth factor receptor tyrosine kinase. Journal of Biological Chemistry. ,vol. 266, pp. 1733- 1739 ,(1991) , 10.1016/S0021-9258(18)52357-7
S. Demignot, M.C. Garnett, R.W. Baldwin, Mouse IgG2b monoclonal antibody fragmentation. Preparation and purification of Fab, Fc and Fab/c fragments. Journal of Immunological Methods. ,vol. 121, pp. 209- 217 ,(1989) , 10.1016/0022-1759(89)90162-2
A B Schreiber, T A Libermann, I Lax, Y Yarden, J Schlessinger, Biological role of epidermal growth factor-receptor clustering. Investigation with monoclonal anti-receptor antibodies. Journal of Biological Chemistry. ,vol. 258, pp. 846- 853 ,(1983) , 10.1016/S0021-9258(18)33127-2
R A Rubin, H S Earp, Solubilization of EGF receptor with Triton X-100 alters stimulation of tyrosine residue phosphorylation by EGF and dimethyl sulfoxide. Journal of Biological Chemistry. ,vol. 258, pp. 5177- 5182 ,(1983) , 10.1016/S0021-9258(18)32555-9
G N Gill, T Kawamoto, C Cochet, A Le, J D Sato, H Masui, C McLeod, J Mendelsohn, Monoclonal anti-epidermal growth factor receptor antibodies which are inhibitors of epidermal growth factor binding and antagonists of epidermal growth factor binding and antagonists of epidermal growth factor-stimulated tyrosine protein kinase activity. Journal of Biological Chemistry. ,vol. 259, pp. 7755- 7760 ,(1984) , 10.1016/S0021-9258(17)42857-2
M J Van de Vijver, R Kumar, J Mendelsohn, Ligand-induced activation of A431 cell epidermal growth factor receptors occurs primarily by an autocrine pathway that acts upon receptors on the surface rather than intracellularly. Journal of Biological Chemistry. ,vol. 266, pp. 7503- 7508 ,(1991) , 10.1016/S0021-9258(20)89475-7
Ulrich Rodeck, Carla Molthoff, Meenhard Herlyn, Michael Varello, Dorothee Herlyn, Zenon Steplewski, Barbara Atkinson, Hilary Koprowski, Tumor Growth Modulation by a Monoclonal Antibody to the Epidermal Growth Factor Receptor: Immunologically Mediated and Effector Cell-independent Effects Cancer Research. ,vol. 47, pp. 3692- 3696 ,(1987)