Involvement of hypoxia-inducible factor-1 α (HIF-1α) in inhibition of benzene on mouse hematopoietic system

摘要: Benzene is an occupational and environmental pollutant that damages the hematopoietic system through oxidant mechanisms. The aims of this study were to assess role oxidation in benzene-mediated damage by determination levels reactive oxygen species (ROS) evaluate hypoxia-inducible factor-1α (HIF-1α) process. C57BL/6 mice exposed benzene at varying concentrations 60, 150, or 300 mg/kg/d for 15 d. Mice groups displayed weight loss, hematologic consequences including decreased red white blood cell counts, reduced platelet count, diminished hemoglobin content, lower number stem cells bone marrow (BM). There was elevated proportional neutrophil count decrease relative thymus weight. In BM there a significant increase ROS 150 mg/kg benzene. However, as result cellular viability, not markedly different between 300-mg/kg dose control, reduced. HIF-1α expression protein all doses conclusion, data indicated may be involved benzene-induced inhibition mouse hematopoiesis oxidative stress play observed toxicity.