Platelet-derived growth factor receptor-beta is induced during tumor development and upregulated during tumor progression in endothelial cells in human gliomas.

摘要: BACKGROUND Endothelial cells proliferate during brain development, are quiescent in normal adult but again under pathologic conditions such as glioma growth. The vascular phenotype of low grade is comparable to brain, however high gliomas focally highly vascularized and there associated prominent endothelial cell proliferation. mechanisms this change unknown evidence that growth factors play an important role process well angiogenesis differentiation. EXPERIMENTAL DESIGN To investigate whether become activated tumorigenesis progression human by a platelet-derived factor (PDGF) dependent pathway, we analyzed receptor-beta (PDGFR-beta) expression situ hybridization immunocytochemistry astrocytoma (grade II), anaplastic oligo-astrocytoma III), glioblastoma multiforme IV). RESULTS PDGFR-beta mRNA was not detectable the vessels expressed vasculature gliomas, particularly proliferations glioblastomas. receptor tumor microvessels, confirmed double immunofluorescence which staining appeared be cells. Primary cultures derived from maintained for 2 days vitro, whereas it vitro brain. Tumor all grades very little situ. CONCLUSIONS Our results indicate malignant glial tumors with upregulation on vascularize tumor. These findings may contribute our understanding regulate vessel differentiation states.