Identification of genes expressed in the migrating primitive myeloid lineage ofXenopus laevis

摘要: BACKGROUND During primitive hematopoiesis in Xenopus, cebpa and spib expressing myeloid cells emerge from the anterior ventral blood island. Primitive migrate throughout embryo are critical for immunity, healing, development. Although definitive has been studied extensively, molecular mechanisms leading to migration of myelocytes remain poorly understood. We hypothesized these have specific extracellular matrix modifying cell motility gene expression. RESULTS In situ hybridization screens transcripts expressed Xenopus foregut mesendoderm at stage 23 identified seven genes with restricted expression cells: destrin; coronin actin binding protein, 1a; formin-like 1; ADAM metallopeptidase domain 28; cathepsin S; tissue inhibitor metalloproteinase-1; protein tyrosine phosphatase nonreceptor 6. A detailed analysis revealed initially aVBI but become dispersed as migratory, similar known markers. Morpholino-mediated loss-of-function mRNA-mediated gain-of-function studies downstream Spib.a Cebpa, key transcriptional regulators lineage. CONCLUSIONS specifically migratory progenitors, providing tools study how different networks operate during development immunity.