ORIGINAL ARTICLE: Follicular Fluid Levels of Interleukin‐10 and Interferon‐gamma do not Predict Outcome of Assisted Reproductive Technologies

摘要: PROBLEM Follicular fluid (FF) and surrounding tissue contains various lymphocytes that synthesize different cytokines. The other sources of cytokines are ovarian somatic cells. FF provides microenvironment for the oocyte immunological factors regulation its development. Changes in expression concentrations certain can influence embryo quality, resulting a reduced ability to implant. Some data shows follicular environment depends on infertility indication. purpose our study was investigate whether interleukin-10 (IL-10) interferon-gamma (IFN-gamma) released human also evaluate impact those fertilization rate, cleavage pregnancy rate. METHODS OF STUDY Couples treated by assisted reproductive technologies were selected this study. A total 121 patients participated Until cytokine detection samples stored at -20 degrees C. In vitro or intracytoplasmic sperm injection procedure used depending After 72 hr, day transfer, morphology evaluated. Embryo transfer based performed. Commercial enzyme-linked immunosorbent assay kit from Diaclone, France quantitative determination IL-10 IFN-gamma FF. Analysis results performed using spss 12.0. RESULTS One hundred twenty-one tested IFN-gamma. detected 14 (11.6%) 108 (89.3%) samples, respectively (range 0.9-31.1 pg/mL 0.7-10.8 IL-10). There no significant correlation between indication, Similarly, did not differ significantly age groups alter CONCLUSION Our showed suitable markers predicting outcome technologies.