Comparison between cytokine concentration in follicular fluid of poor and high responder patients and their influence of ICSI-outcome.
作者： M. E. Hammadeh , C. Fischer-Hammadeh , T. Georg , P. Rosenbaum , W. Schmidt
DOI: 10.1034/J.1600-0897.2003.00062.X
关键词:
摘要: OBJECTIVE The aim of this study was to compare the cytokine concentration in follicular fluid (FF) low and high responder intracytoplasmic sperm injection (ICSI) patients find out impact these cytokines FF on ICSI outcome. DESIGN levels insulin-like growth factor (IGF)-I, IL-6, IL-8, epidermal (EGF), platelet-derived (PDGF), granulocyte-macrophage-colony stimulating (GM-CSF) were measured from patients, results compared between two groups their influence outcome analysed. MATERIAL AND METHODS A total 49 (G.I) 34 (G.II) enrolled study. collected at time oocyte retrieval either by enzyme-linked immunosorbent assay (IL-6, IL8, EGF, PDGF, GM-CSF) or radio immuno (IGF-I). RESULTS IL-6 (pg/mL), IL-8 IGF-I (ng/mL), PDGF EGF GM-CSF (pg/mL) G.I 6.0 +/- 4.3, 288.1 139.2, 0.416 0.089, 249.8 150.1, 9.12 5.5 1.45 2.10 corresponding value G.II 7.4 4.8, 208.6 64.0, 0.431 0.094, 387.6 36.0, 8.9 5.4 1.8 3.3, respectively. Only showed a significant (P = 0.007) difference groups. Besides, negative correlations found fertilization rate (r -0.287; P 0.046) G.I. mean number retrieved (6.4 2.3 versus. 15.7 5.4) fertilized (3.6 1.6 versus 7.0 4.5) oocytes differ significantly 0.001) higher than (60.9 25.1 43.4 20.7%). CONCLUSION There no IGF-I, concentrations patients. with Moreover, poor correlation rate. However, undergoing controlled ovarian hyperstimulation for could not be used as marker implantation potential.
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