Allelic Imbalance Is a Prevalent and Tissue-Specific Feature of the Mouse Transcriptome

摘要: In mammals, several classes of monoallelic genes have been identified, including those subject to X-chromosome inactivation (XCI), genomic imprinting, and random expression (RMAE). However, the extent which these epigenetic phenomena are influenced by underlying genetic variation is unknown. Here we perform a systematic classification allelic imbalance in mouse hybrids derived from reciprocal crosses divergent strains. We observe that deviation balanced biallelic common, occurring ∼20% transcriptome given tissue. Allelic attributed genotypic far most prevalent class typically tissue-specific. some genotype-based maintained across tissues associated with greater variation, especially 5′ 3′ termini transcripts. further identify novel imprinted find genotype can modify penetrance parental origin even setting large regions. Examination nascent transcripts single cells inbred strains reveals showing also exhibit isogenic backgrounds. This surprising observation may suggest competition between alleles and/or reflect combined impact cis- trans-acting on gene. Our findings provide insights into gene regulation be relevant human disease.