Employment of methylene blue irradiated with laser light source in photodynamic inactivation of biofilm formed by Candida albicans strain resistant to fluconazole
作者： Lucia Černáková , Stanislava Dižová , Helena Bujdáková
DOI: 10.1093/MMY/MYW137
关键词:
摘要: A promising approach for the eradication of biofilm formed by yeast Candida albicans seems to be photodynamic inactivation (PDI). This work presents a use methylene blue (MB, 1 mM) irradiated with red laser (output power 190 mW/cm2, wavelength 660 nm) fluconazole-resistant (FLC-resistant) strain C. CY 1123 compared standard SC5314. The periods irradiation corresponded fluence 15, 23 and 57 J/cm2. Effectiveness PDI was evident following percentage survived cells: 24.57, 23.46, 22.29% SC5314 40.28, 17.91, 5.89% 1123, respectively, samples without irradiation. Light confocal scanning microscopy confirmed effectiveness PDI. However, morphological form play an important role as well, since prolonged duration did not increase efficiency on An experiment yeast-to-hyphae transition revealed that FLC-resistant expressed markedly reduced capacity hyphae We summarized effective strain, but resistance most likely significant in Additionally, we observed differences susceptibility between biofilms composed mycelia only yeasts, finally, employment enabled decreasing period while maintaining high
oup.com
sci-hub.se 参考文章(28)
Mohd. Asif Sherwani, Saba Tufail, Aijaz Ahmed Khan, Mohammad Owais, Gold Nanoparticle-Photosensitizer Conjugate Based Photodynamic Inactivation of Biofilm Producing Cells: Potential for Treatment of C. albicans Infection in BALB/c Mice PLOS ONE. ,vol. 10, pp. e0131684- ,(2015) , 10.1371/JOURNAL.PONE.0131684
Lívia Nordi Dovigo, Ana Cláudia Pavarina, Ewerton Garcia de Oliveira Mima, Eunice Teresinha Giampaolo, Carlos Eduardo Vergani, Vanderlei Salvador Bagnato, Fungicidal effect of photodynamic therapy against fluconazole-resistant Candida albicans and Candida glabrata Mycoses. ,vol. 54, pp. 123- 130 ,(2011) , 10.1111/J.1439-0507.2009.01769.X
Lucia Černáková, Jaroslava Chupáčová, Katarína Židlíková, Helena Bujdáková, Effectiveness of the Photoactive Dye Methylene Blue versus Caspofungin on the Candida parapsilosis Biofilm in vitro and ex vivo Photochemistry and Photobiology. ,vol. 91, pp. 1181- 1190 ,(2015) , 10.1111/PHP.12480
Juliana Pereira Lyon, Leonardo Marmo Moreira, Pedro Claudio Guaranho de Moraes, Fábio Vieira dos Santos, Maria Aparecida de Resende, Photodynamic therapy for pathogenic fungi. Mycoses. ,vol. 54, ,(2011) , 10.1111/J.1439-0507.2010.01966.X
P. Robres, C. Aspiroz, A. Rezusta, Y. Gilaberte, Utilidad de la terapia fotodinámica en el manejo de la onicomicosis Actas Dermo-Sifiliográficas. ,vol. 106, pp. 795- 805 ,(2015) , 10.1016/J.AD.2015.08.005
M. Tamura, K. Watanabe, Y. Mikami, K. Yazawa, K. Nishimura, Molecular characterization of new clinical isolates of Candida albicans and C. dubliniensis in Japan: analysis reveals a new genotype of C. albicans with group I intron. Journal of Clinical Microbiology. ,vol. 39, pp. 4309- 4315 ,(2001) , 10.1128/JCM.39.12.4309-4315.2001
Helena Bujdáková, Tomáš Kuchta, Eva Sidóová, Anna Gvozdjaková, Anti-Candida activity of four antifungal benzothiazoles Fems Microbiology Letters. ,vol. 112, pp. 329- 333 ,(1993) , 10.1111/J.1574-6968.1993.TB06471.X
Lingmei Sun, Kai Liao, Dayong Wang, Effects of Magnolol and Honokiol on Adhesion, Yeast-Hyphal Transition, and Formation of Biofilm by Candida albicans PLOS ONE. ,vol. 10, ,(2015) , 10.1371/JOURNAL.PONE.0117695
Reza Fekrazad, Vadood Ghasemi Barghi, Arash Poorsattar Bejeh Mir, Masoumeh Shams-Ghahfarokhi, In vitro photodynamic inactivation of Candida albicans by phenothiazine dye (new methylene blue) and Indocyanine green (EmunDo Photodiagnosis and Photodynamic Therapy. ,vol. 12, pp. 52- 57 ,(2015) , 10.1016/J.PDPDT.2014.12.006
T.S. Mang, L. Mikulski, R.E. Hall, Photodynamic inactivation of normal and antifungal resistant Candida species. Photodiagnosis and Photodynamic Therapy. ,vol. 7, pp. 98- 105 ,(2010) , 10.1016/J.PDPDT.2010.03.001