Combined analysis of microsatellite instability and K-ras mutation increases detection incidence of normal samples from colorectal cancer patients.
作者： H Esumi , A Ochiai , T Minamoto , G Belitsky , T Sugimura
DOI:
关键词:
摘要: Microsatellite instability (MI) and K-ras oncogene mutation have been widely used as biomarkers of genetic changes in colorectal cancer (CRC). Each these was independently found normal-appearing colonic mucosa at stages preceding the development CRC, albeit a relatively low incidence. To assess potential value combined MI analysis detection samples taken from patients with we chosen to analyze multiple (3-7) normal respective tumor tissues 20 CRC. As control, 54 obtained 9 autopsies without In least 1 5 loci analyzed, 8 via each patient. Combined mutant ras alleles enabled identification 11 None CRC were carry or MI. The ability detect 55% provides an important advance our approach toward early individuals who may be risk develop this type.
aacrjournals.org参考文章(19)
Gabor Gyapay, Jean Morissette, Alain Vignal, Colette Dib, Cécile Fizames, Philippe Millasseau, Sophie Marc, Giorgio Bernardi, Mark Lathrop, Jean Weissenbach, The 1993-94 Généthon human genetic linkage map. Nature Genetics. ,vol. 7, pp. 246- 339 ,(1994) , 10.1038/NG0694SUPP-246
M. Tohi, F.-C. Luo, Z. Ronai, Detection of K-ras Mutation in Colonic Effluent Samples From Patients Without Evidence of Colorectal Carcinoma Journal of the National Cancer Institute. ,vol. 86, pp. 1007- 1010 ,(1994) , 10.1093/JNCI/86.13.1007
Yurij Ionov, Miguel A. Peinado, Sergei Malkhosyan, Darryl Shibata, Manuel Perucho, Ubiquitous somatic mutations in simple repeated sequences reveal a new mechanism for colonic carcinogenesis Nature. ,vol. 363, pp. 558- 561 ,(1993) , 10.1038/363558A0
Bert Vogelstein, Kenneth W. Kinzler, The multistep nature of cancer. Trends in Genetics. ,vol. 9, pp. 138- 141 ,(1993) , 10.1016/0168-9525(93)90209-Z
Eric R. Fearon, Bert Vogelstein, A genetic model for colorectal tumorigenesis Cell. ,vol. 61, pp. 759- 767 ,(1990) , 10.1016/0092-8674(90)90186-I
E. Stanbridge, Identifying Tumor Suppressor Genes in Human Colorectal Cancer Science. ,vol. 247, pp. 12- 13 ,(1990) , 10.1126/SCIENCE.2403692
L. Mao, M. P. Schoenberg, M. Scicchitano, Y. S. Erozan, A. Merlo, D. Schwab, D. Sidransky, Molecular Detection of Primary Bladder Cancer by Microsatellite Analysis Science. ,vol. 271, pp. 659- 662 ,(1996) , 10.1126/SCIENCE.271.5249.659
Z RONAI, FC LUO, S GRADIA, WJ HART, R BUTLER, Detection of k-ras mutation in normal and malignant colonic tissues by an enriched PCR method. International Journal of Oncology. ,vol. 4, pp. 391- 396 ,(1994) , 10.3892/IJO.4.2.391
Marina S. Yakubovskaya, Vladimir Spiegelman, Feng C. Luo, Serge Malaev, Alexander Salnev, Irina Zborovskaya, Alexander Gasparyan, Boris Polotsky, Zurab Machaladze, Alexander C. Trachtenberg, Gennady A. Belitsky, Zeev Ronai, High frequency of K-ras mutations in normal appearing lung tissues and sputum of patients with lung cancer. International Journal of Cancer. ,vol. 63, pp. 810- 814 ,(1995) , 10.1002/IJC.2910630611
S. Baker, E. Fearon, J. Nigro, Hamilton, A. Preisinger, J. Jessup, P vanTuinen, D. Ledbetter, D. Barker, Y Nakamura, R White, B Vogelstein, Chromosome 17 deletions and p53 gene mutations in colorectal carcinomas Science. ,vol. 244, pp. 217- 221 ,(1989) , 10.1126/SCIENCE.2649981