Sensitive detection of K-ras mutations augments diagnosis of colorectal cancer metastases in the liver.

摘要: Postoperative survival of colorectal cancer patients is often delineated by metastases spreading to the liver. Current clinical diagnostic procedures are unable discover micrometastases in this organ. Our aim was develop a tool for detecting that present at time surgery. Therefore, PCR-RFLP assay set up tracking point mutations K-ras oncogene codons 12 and 13, based on mismatch primers restriction enzymes BstXI XcmI. The detection limit one mutant million wild-type cells. One hundred forty-two with carcinoma were screened these tissue samples from their tumor, proximally adjacent mucosa, Of these, 67 (46%) positive mutation, which 58 had codon 9 13 mutations. No patient without K-ras-positive tumor showed mutation but 11 (11 58; 19%) found bear 21 (21 64; 33%), detected liver tissue. Sequencing all mutated revealed 92% confirmation results. In summary, useful allows early proof molecular determinants metastases. Such knowledge will improve staging could beneficially influence prognosis if followed an effective therapy.