K-ras mutations in sporadic colorectal tumors in Israel: unusual high frequency of codon 13 mutations and evidence for nonhomogeneous representation of mutation subtypes.

摘要: Colorectal cancer is one of the most common malignancies in western world, including Israel. An important step progression includes induction activating mutations protooncogene K-ras. This event very frequent and detected about 40% colorectal carcinomas. Previous studies a variety genetic disorders revealed unique gene mutation prevalence Jewish populations, attributed both to differences background variability environmental exposure. To determine incidence molecular subtypes K-ras Israel, compared with other countries, DNA was isolated from random collection 105 carcinoma samples, were by an improved designed RFLP direct sequencing. sporadic Israel are relatively frequent, higher fraction codon 13 than reported thus far. Comparison countries shows vast heterogeneity terms relative abundance affected type representation specific mutations. The heterogeneous distribution found may be due variable different factors involved initiation propagation cancer.