K-ras Mutations and Prognosis in Large-Bowel Carcinomas

摘要: Background: Colorectal carcinogenesis is regarded as a multistep process involving several genetic alterations, with mutation in the K-ras gene about half of tumours. We aimed at clarifying role this alteration related to survival and clinicopathologic variables. Methods: One hundred large-bowel carcinomas operated on between 1978 1982 were studied for presence point mutations codons 12 13 gene, using enriched polymerase chain reaction amplification. restriction fragment length polymorphism analysis, direct sequencing. Results: Forty found (40%); 31 codon 9 13, 7 different types. There was no relationship tumours without regard Dukes' stages, age or sex patient, tumour localization, histologic grade, DNA ploidy pattern, HLA-DR staining survival. Samples from 5 localizations showed identical did 19 recurre...