DNA repair genes in human fetal and adult prostatic tissues and cancer cell lines using differential RT-PCR
作者： Rajvir Dahiya
DOI: 10.1016/S1078-1439(96)00048-8
关键词:
摘要: The present study was designed to investigate the mRNA expression of four DNA repair genes (XPCC, hMSH2, XRCC 1, and ERCC 1) in human fetal adult prostatic tissues cancer cell lines using differential reverse transcriptase-polymerase chain reaction (RT-PCR). For this purpose, total RNA from prostrate (LNCaP, PC-3, DU-145, ND-I) (n = 10) prostates extracted analyzed for by RT-PCR specific oligonucleotides. quantitation, we used β-actin as an internal standard each tube baseline gene expression. results these experiments suggest that prostate have 2- 10-fold lower all compared with tissues. Expression XPCC about 10- 15-fold cells This study, first time, demonstrates levels are significantly prostate.
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