High frequency of genetic instability of microsatellites in human prostatic adenocarcinoma.
作者： Rajvir Dahiya , Celeste Lee , Joseph McCarville , Weixing Hu , Gurjit Kaur
DOI: 10.1002/(SICI)1097-0215(19970904)72:5<762::AID-IJC10>3.0.CO;2-B
关键词:
摘要: In order to investigate the genomic instability associated with prostate cancer, 36 microsatellite marker loci on chromosomes 1p, 3p, 5q, 8p, 8q, 9p, 11q and 13q were analyzed using microdissected samples from cancer adjoining microscopically normal tissues same slide. DNA was extracted tumor cells of 40 prostate-cancer samples, amplified by PCR, for (MSI) different polymorphic markers. present study, we have utilized a highly refined technique PCR product separation sequencing gel, developed in our laboratory, which clearly shows high-quality results cancer. The this study suggest that 45% (18 out 40) showed at minimum 1 locus; 4 cases each MSI one 2 loci, had 3 5 while case 7, 8 15 loci. There no significant correlation between stage or grade tumors. This extensive found occurrence be very high, suggests role pathophysiology
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