Multipotent stem cells derived from placenta tissue and cellular therapeutic agents comprising the same
作者： Bong Hui Kim , Hanna Park , Hyoeun Kim , Sang Kyu Woo , Hang Young Lee
DOI:
关键词:
摘要: The present invention relates to placenta tissue-derived multipotent stem cells and cell therapeutic agents containing the same. More specifically, a method for producing having following characteristics, comprising culturing amnion, chorion, decidua or tissue in medium collagenase bFGF collecting cultured cells: (a) showing positive immunological response CD29, CD44, CD73, CD90 CD105, negative CD31, CD34, CD45 HLA-DR; (b) Oct4 SSEA4; (c) growing attached plastic, round-shaped spindle-shaped morphology, forming spheres an SFM so as be able maintained undifferentiated state long period of time; (d) ability differentiate into mesoderm-, endoderm- ectoderm-derived cells. Also obtained using production method. inventive have muscle cells, vascular endothelial osteogenic nerve satellite fat cartilage-forming insuline-secreting pancreatic β-cells, thus are effective treatment muscular diseases, osteoporosis, osteoarthritis, nervous diabetes like, useful formation breast tissue.
lens.org参考文章(12)
Konstantinos Stefanidis, Compositions and methods for identifying, isolating and enriching germline-like stem cells from amniotic fluid ,(2007)
Yuan-Di C. Halvorsen, William O. Wilkison, Jeffrey M. Gimble, Christopher Luft, Bentley Cheatham, Methods and compositions for the use of stromal cells to support embryonic and adult stem cells ,(2002)
Gail K. Naughton, Compositions and methods for naturally secreted extracellular matrix ,(1996)
Stephen C. Strom, Toshio Miki, Placental derived stem cells and uses thereof ,(2003)
Samy Ashkar, Anthony Atala, Injectable non-immunogenic cartilage and bone preparation ,(1995)
Jin-Lian Tsai, Kung-Kai Kuo, Tsai-Ming Lin, Chia-Cheng Chang, Adult stem cells and uses thereof ,(2005)
C. Bettina Portmann-Lanz, Andreina Schoeberlein, Alexander Huber, Ruth Sager, Antoine Malek, Wolfgang Holzgreve, Daniel V. Surbek, Placental mesenchymal stem cells as potential autologous graft for pre- and perinatal neuroregeneration American Journal of Obstetrics and Gynecology. ,vol. 194, pp. 664- 673 ,(2006) , 10.1016/J.AJOG.2006.01.101
T MATIKAINEN, J LAINE, Placenta--an alternative source of stem cells. Toxicology and Applied Pharmacology. ,vol. 207, pp. 544- 549 ,(2005) , 10.1016/J.TAAP.2005.01.039
James Edinger, Qian Ye, Jai-Lun Wang, Robert J. Hariri, Placental stem cell populations ,(2006)
Anthony Atala, Keith T. Paige, Linda Griffith-Cima, Charles A. Vacanti, Tissue formation by injecting a cell-polymeric solution that gels in vivo ,(1993)