Factors Affecting Adventitous Shoot Regeneration "in vitro" in the Apple Rootstock 'Jork9'
作者： M. Tonelli , E. Caboni , C. Damiano , G. Falasca
DOI: 10.1400/75447
关键词:
摘要: The regeneration of adventitious shoots on leaf segments taken from in vitro the apple rootstock 'Jork 9' was examined. Among macroelements and organics tested (MS, LS or N?), MS most effective. best combination naphthaleneacetic acid (NAA) benzyladenine (BA) 2.7 pM 17.8 pM, respectively, resulting 70% expiants with shoots. 0.1 thidiazuron (TDZ) also resulted a high percentage but almost all were hyperhydric. Only adaxial surface contact medium showed regeneration. Basal within had higher than distal expiants. Cell divisions, localized near vascular bundles, observed after three days culture. first vegetative buds regenerated directly tissue, whereas that appeared later callus.
jstor.org 
sci-hub.st 参考文章(12)
Luz Marcela Yepes, Herb S. Aldwinekle, Factors that effect leaf regeneration efficiency in apple, and effect of antibiotics in morphogenesis Plant Cell Tissue and Organ Culture. ,vol. 37, pp. 257- 269 ,(1994) , 10.1007/BF00042339
Ching-San Sun, Chih-Ching Chu, Ching-Chu Wang, Chen Hsu, Chih--Yin Chu, Kwang-Chu Yin, Feng-Yun Bi, ESTABLISHMENT OF AN EFFICIENT MEDIUM FOR ANTHER CULTURE OF RICE THROUGH COMPARATIVE EXPERIMENTS ON THE NITROGEN SOURCES Science of Sintering. ,vol. 18, pp. 659- 668 ,(1975) , 10.1360/YA1975-18-5-659
William A. Jensen, Botanical Histochemistry: Principles and Practice ,(2015)
Elfriede M. Linsmaier, Folke Skoog, Organic Growth Factor Requirements of Tobacco Tissue Cultures Physiologia Plantarum. ,vol. 18, pp. 100- 127 ,(1965) , 10.1111/J.1399-3054.1965.TB06874.X
Margareta Welander, Plant Regeneration from Leaf and Stem Segments of Shoots Raised in vitro from Mature Apple Trees Journal of Plant Physiology. ,vol. 132, pp. 738- 744 ,(1988) , 10.1016/S0176-1617(88)80238-4
Carl A. Huetteman, John E. Preece, Thidiazuron: a potent cytokinin for woody plant tissue culture Plant Cell Tissue and Organ Culture. ,vol. 33, pp. 105- 119 ,(1993) , 10.1007/BF01983223
S. Predieri, F. Fasolo Fabbri Malavasi, High-frequency shoot regeneration from leaves of the apple rootstock M26 ( Malus pumila Mill.) Plant Cell Tissue and Organ Culture. ,vol. 17, pp. 133- 142 ,(1989) , 10.1007/BF00046858
Toshio Murashige, Folke Skoog, A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures Physiologia Plantarum. ,vol. 15, pp. 473- 497 ,(1962) , 10.1111/J.1399-3054.1962.TB08052.X
RB Horsch, JE Fry, NL Hoffmann, M Wallroth, D Eichholtz, SG Rogers, RT Fraley, A simple and general method for transferring genes into plants Science. ,vol. 227, pp. 1229- 1231 ,(1985) , 10.1126/SCIENCE.227.4691.1229
David J. James, Andrew J. Passey, Eddo Rugini, Factors Affecting High Frequency Plant Regeneration from Apple Leaf Tissues Cultured in vitro Journal of Plant Physiology. ,vol. 132, pp. 148- 154 ,(1988) , 10.1016/S0176-1617(88)80152-4