Characterization of a family of rhoptry proteins of Toxoplasma gondii.
作者： A SADAK , Z TAGHY , B FORTIER , J DUBREMETZ
DOI: 10.1016/0166-6851(88)90075-8
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摘要: Abstract A monoclonal antibody (McAb 4A7) raised against a rhoptry enriched subcellular fraction of Toxoplasma gondii tachyzoites reacted in immunofluorescence studies with rod-like organelles located the anterior part organisms and gave specific labeling rhoptries immunoelectron microscopy. On immunoblots, two major proteins 55 60 kDa were identified by McAb 4A7. Similar results obtained both immunodetection immonoblotting tachyzoites, bradyzoites sporozoites. Pulse chase analysis [ 35 S]methionine labeled demonstrated that derived from 66–68 doublet which was processed approximately 30 min after biosynthesis. Two other antibodies 2F8, 2H3) respectively for having 66 precursor 68 also obtained; we suggest they recognize separately components 55–60 protein family .
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