Entactin-2: a new member of basement membrane protein with high homology to entactin/nidogen.
作者： Naoki Kimura , Tomoko Toyoshima , Tetsuo Kojima , Miyuki Shimane
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摘要: Abstract Using the new signal sequence trap (SST) method, we isolated several clones encoding secreted and transmembrane proteins from KUSA cells, a murine osteoblast-like cell line. One novel clone, termed entactin-2, exhibited high similarity to mouse entactin/nidogen, basement membrane protein. Although deduction of amino acid entactin-2 revealed only 27.4% homology entactin, many structural similarities were seen between both proteins. Entactin-2 contains five EGF-like two thyroglobulin-like motifs, which are cysteine-rich. Comparison clearly that also related domain structures. The rod-like containing RGD integrin recognition sequence, fused glutathione-Stransferase (GST), surface-binding activity similar entactin. In addition, tissue distribution mRNA resembled Furthermore, expression genes decreased as osteoblastic differentiation progressed. These results suggest is member entactin gene family, may have entactin-related functions, might act component.
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