Synergistic cooperation of TFE3 and smad proteins in TGF-beta-induced transcription of the plasminogen activator inhibitor-1 gene.
作者： X. Hua , X. Liu , D. O. Ansari , H. F. Lodish
关键词:
摘要: Members of the TGF-β superfamily influence a broad range biological activities including stimulation wound healing and inhibition cell growth. signals through type I II receptor serine/ threonine kinases induces transcription many genes plasminogen activator inhibitor-1 (PAI-1). To identify proteins that participate in TGF-β-induced gene expression, we developed novel retrovirus-mediated expression cloning strategy; using this approach, established factor μE3 (TFE3) is involved activation PAI-1 promoter. We showed TFE3 binds to an E-box sequence PE2, 56-bp promoter fragment promoter, mutation abolishes both binding as well TGF-β-dependent activation. Smad3 synergistically activate PE2 phosphorylated Smad4 bind adjacent TFE3-binding site Binding Smad their cognate sequences indispensable for TGF-β-inducible Hence, important at least one TGF-β-activated signal transduction pathway.
genesdev.org参考文章(43)
L L Lanier, A Miyajima, G P Nolan, A Shibuya, Y Morikawa, T Kitamura, J Phillips, S Kinoshita, M Onishi, D M Gorman, Applications of retrovirus-mediated expression cloning. Experimental Hematology. ,vol. 24, pp. 324- 329 ,(1996)
Suraiya Rasheed, Retroviruses and Oncogenes Springer, Boston, MA. pp. 293- 408 ,(1995) , 10.1007/978-1-4899-1721-8_5
Liliana Attisano, Jeffrey L. Wrana, Fernando López-Casillas, Joan Massagué, TGF-beta receptors and actions. Biochimica et Biophysica Acta. ,vol. 1222, pp. 71- 80 ,(1994) , 10.1016/0167-4889(94)90026-4
R. Wieser, J.L. Wrana, J. Massagué, GS domain mutations that constitutively activate T beta R-I, the downstream signaling component in the TGF-beta receptor complex. The EMBO Journal. ,vol. 14, pp. 2199- 2208 ,(1995) , 10.1002/J.1460-2075.1995.TB07214.X
M.R. Keeton, S.A. Curriden, A.J. van Zonneveld, D.J. Loskutoff, Identification of regulatory sequences in the type 1 plasminogen activator inhibitor gene responsive to transforming growth factor beta. Journal of Biological Chemistry. ,vol. 266, pp. 23048- 23052 ,(1991) , 10.1016/S0021-9258(18)54461-6
Jeffrey L Wrana, Liliana Attisano, Rotraud Wieser, Francesc Ventura, Joan Massagué, None, Mechanism of activation of the TGF-β receptor Nature. ,vol. 370, pp. 341- 347 ,(1994) , 10.1038/370341A0
Xin Chen, Ellen Weisberg, Valerie Fridmacher, Minoru Watanabe, Grace Naco, Malcolm Whitman, Smad4 and FAST-1 in the assembly of activin-responsive factor. Nature. ,vol. 389, pp. 85- 89 ,(1997) , 10.1038/38008
J J Billadello, W E Hopkins, D R Westerhausen, Multiple transforming growth factor-beta-inducible elements regulate expression of the plasminogen activator inhibitor type-1 gene in Hep G2 cells. Journal of Biological Chemistry. ,vol. 266, pp. 1092- 1100 ,(1991) , 10.1016/S0021-9258(17)35287-0
Jaeseob Kim, Kirby Johnson, Hui Ju Chen, Sean Carroll, Allen Laughon, Drosophila Mad binds to DNA and directly mediates activation of vestigial by Decapentaplegic Nature. ,vol. 388, pp. 304- 308 ,(1997) , 10.1038/40906
Carl-Henrik Heldin, Kohei Miyazono, Peter ten Dijke, TGF-beta signalling from cell membrane to nucleus through SMAD proteins Nature. ,vol. 390, pp. 465- 471 ,(1997) , 10.1038/37284