Nucleotide sequence of the Drosophila glucose-6-phosphate dehydrogenase gene and comparison with the homologous human gene.

摘要: Glucose-6-phosphate dehydrogenase (G6PD) has a major role in NADPH production and is found almost all cell types. The structural gene for G6PD X-linked Drosophila melanogaster, as it most eukaryotic organisms, due to its ubiquitous expression, can be considered typical 'housekeeping' gene. Here we present the complete nucleotide (nt) sequence of cDNAs well genomic copy three introns so that protein-coding region divided into four segments. 5'-end mature mRNA located 289 +/- 1 nt upstream from start codon. transcription point G + T-rich contains no commonly regulatory elements, such TATA box or GGGCGG sequence. D. melanogaster 65% homologous with human protein but homology first 42 amino acid residues. was shown active when transduced autosomal positions. For each transformant, activity both male female adults not significantly different, indicating gene, unlike resident G6PD, dosage-compensated males.