ELISA methods comparison for the detection of auto-antibodies against apolipoprotein A1.

摘要: Abstract Background Autoantibodies against apolipoprotein A1 (anti-apoA1 IgG) have emerged as an independent biomarker for cardiovascular disease and mortality. Across studies, different ELISA methods been used to measure the level of circulating anti-apoA1 IgG which could lead substantial result differences between assays. Objectives To make a comparative study available detection determine whether choice matrix sample (serum vs plasma) influence results. Methods Blood samples were obtained from 160 healthy blood donors collected on 4 matrixes (serum, plasma-EDTA, -citrate, -lithium-heparinate). Anti-apoA1 was measured using two homemade (Geneva's Lisbon's) one commercial kits. Passing-Bablok Bland-Altman compare seropositivity cut-offs defined according user's/manufacturer's criterion. Results The current results showed those 3 dynamic ranges significantly different, kit displaying narrowest one. analysis demonstrated important proportional constant biases rate in Geneva, Lisbon assays varied 24.5% 1.9%. Matrix comparisons that (plasma versus serum) influenced well assay-dependent manner. coating antigen source identified factor underlying heterogeneity across Conclusions These highlight impact method cut-off emphasize need standardizing existing Given influence, we suggest use serum choice.