Binding of mouse and rabbit iron-59 transferrins to lactating mouse mammary epithelial cells.

摘要: Abstract The binding of mouse and rabbit transferrins to lactating mammary epithelial cells was tested in a 59 Fe-protein-binding assay. homologous heterologous slow during the first 30min, after which uptake steadily increased. In ligand concentration-dependent saturation studies, protein showed high degree required approximately 9.7ng saturate ∼2 x 10 6 cells. demonstrated low failed demonstrate at above concentration. Scatchard plot for data nonlinear implied (1.08 –10 M ) (1.82 –9 affinity interaction mechanism. However, linear characterized by one (1.0 interaction. A total 11,000 19,600 sites per cell were estimated proteins, respectively. These suggest crossreactivity between transferrins. mechanism seems be conserved proteins from both species; however, an additional present only system.