Flow karyotyping and chromosome sorting

摘要: For flow karyotyping and sorting, chromosomes are stained in suspension with appropriate DNA fluorochromes, passed singly through a beam of laser light. The emitted pulses fluorescence scattered light measured stored the form histogram displaying number versus or intensity. A data processing system was devised to remove signals from cell debris chromosome fragments. selective window set dot plot intensities program made count gated area only. As application fractionation, cytometry has been used for karyotype analysis, gene mapping, chromosomal library construction.