Continuous human bone marrow culture: Ia antigen characterization of probable pluripotential stem cells

摘要: The presence of Ia-like antigens on human CFU-C and BFU-e is confirmed a cell type that lacked immediate capacity for granulocytic colony formation but generated CFU-c after brief incubation in simple suspension culture identified. This pre-CFU-c, its progeny, was extremely sensitive to killing by anti-Ia serum with complement. In contrast, plus complement treatment bone marrow, while eliminating 93%-97% all BFU-e, did not prevent the rapid regeneration these progenitor cells their production some weeks under conditions continuous marrow culture. These studies suggest equivalent pluripotential stem can replicate generate committed progenitors, such as BFU-e. Furthermore, it would appear antigen absent cell, rapidly gained commitment various types occur, subsequently lost latter undergo differentiation within marrow.