Comparison of Sanger Sequencing, Pyrosequencing, and Melting Curve Analysis for the Detection of KRAS Mutations
作者： Athanasios C. Tsiatis , Alexis Norris-Kirby , Roy G. Rich , Michael J. Hafez , Christopher D. Gocke
DOI: 10.2353/JMOLDX.2010.090188
关键词: DNA Mutational Analysis 、 Genetics 、 Mutation 、 KRAS 、 Pyrosequencing 、 Biology 、 Molecular biology 、 Allele 、 Melting curve analysis 、 Sanger sequencing 、 False positive paradox 、 Pathology and Forensic Medicine 、 Molecular medicine
摘要: … Finally, using an electronic cutoff of 10%, Sanger sequencing … for Sanger sequencing, pyrosequencing, and melting curve analysis was approximately 15 to 20%, 5%, and 10% mutant …
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