Assessment of tobacco heating product THP1.0. Part 5: In vitro dosimetric and cytotoxic assessment.
作者： Tomasz Jaunky , Jason Adamson , Simone Santopietro , Anya Terry , David Thorne
DOI: 10.1016/J.YRTPH.2017.09.016
关键词: In vitro 、 Statistical difference 、 Toxicant 、 Weight of evidence 、 Toxicology 、 Chemistry 、 Neutral red 、 Cytotoxic T cell 、 Food science 、 Tobacco smoke 、 Nicotine
摘要: Abstract Tobacco heating products (THPs) represent a subset of the next-generation nicotine and tobacco product category, in which is typically heated at temperatures 250–350 °C, thereby avoiding many harmful combustion-related toxicant emissions conventional cigarettes. In this study, we have assessed aerosol generation cytotoxicity from two commercially available THPs, THP1.0 THS, relative to smoke 3R4F reference cigarettes, using an adapted Borgwaldt RM20S Smoking Machine. Quantification exposed cell-culture media showed greater delivery both THPs than cigarette. Using Neutral Red Uptake assay, demonstrated reduced in vitro H292 human bronchial epithelial cells as compared with cigarette exposure air–liquid interface (p 87% viability common dilution (1:40, aerosol:air). A similar response was observed when plotted against nicotine; statistical difference between biological testing forms part larger package data help assess safety risk reduction potential weight evidence approach.
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