Identification of Defensin α6 as a Potential Biomarker in Colon Adenocarcinoma

摘要: Abstract There is substantial interest in the identification of circulating human tumor-derived proteins serum for purposes early cancer diagnosis. We have implemented an approach based on analysis microarray data tumor that may utility as biomarkers colon cancer. Expression obtained from a variety 283 tumors and normal tissues revealed defensin α6 was maximally expressed These findings were corroborated by reverse transcription-PCR, which cell lines LoVo, Caco2, HCT-15, SW480, SW620 showed significantly higher levels expression than did non-colon lines. Moreover, our concordant with NCI, National Institutes Health Cancer Genome Anatomy Project. To evaluate potential biomarker cancer, preliminary “training” set 91 healthy donors 109 patients analyzed enzyme-linked immunosorbent assay. The pattern confirmed independent 67 masked samples: 18 49 patients. This result yielded sensitivity 69.4% (95% CI 54.6–81.8), specificity 83.3% (58.6–96.4), positive predictive value 91.9% (78.1–98.3). justify prospective assessment protein screening tool