Control of programmed cell death by the baculovirus genes p35 and iap.

摘要: The SF-21 insect cell line undergoes rapid and widespread apoptosis when treated with actinomycin D or infected a mutant of the baculovirus Autographa californica nuclear polyhedrosis virus lacking p35 gene functionally active iap (inhibitor apoptosis) gene. Here we provide evidence that basis for induction by these two different stimuli is cessation RNA synthesis. We also show expression either functional homologs blocks independently other viral genes, indicating products act directly on cellular apoptotic pathway. genes encode C3HC4 (or RING) finger motif found in number transcriptional regulatory proteins, as well additional Cys/His motifs (baculovirus repeats). specific amino acids within both N-terminal repeat are critical anti-apoptosis function. Overexpression mammalian bcl-2 adenovirus E1B-19K, which block overexpressed cells, does not D-induced cells.