Population-based characterization of the genetic landscape of chronic lymphocytic leukemia patients referred for cytogenetic testing in British Columbia, Canada: the role of provincial laboratory standardization

摘要: Detection of recurrent chromosome abnormalities by fluorescence in situ hybridization (FISH) is an essential component care chronic lymphocytic leukemia (CLL) patients. In the province British Columbia (BC), Canada, population 4.6 million, CLL patients receive uniform evaluation and therapy with FISH testing performed three jurisdictions. The aims this study were to (i) validate CLL-FISH among BC cytogenetic laboratories ensure standardization results (ii) characterize population-level pooling provincial data. From 2004 2011, 585 consecutive underwent pretreatment at laboratory A (50.1%), B (32.3%), or C (17.6%). For validation purposes, 26 tested each laboratory's protocol, 91% result concordance. Discordant involved percent near cutoff values; therefore, a 10% universal was established when results. Applying cohort, detected 74.9%: 54.9% 13q-, 18.8% +12, 8.5% 11q-, 7.7% 17p-. large population-based cohort referred for testing, frequencies analysis highly consistent those reported single-institution clinical trial populations. Provinces districts that work together can effectively pool data appropriate