The protective effect of follicular fluid against the emerging mycotoxins alternariol and beauvericin
作者： R.R. Santos , E.J. Schoevers , X. Wu , B.A.J. Roelen , J. Fink-Gremmels
DOI: 10.3920/WMJ2014.1829
关键词: Alternariol 、 Trolox equivalent antioxidant capacity 、 Follicular fluid 、 Oxidative stress 、 Beauvericin 、 Molecular biology 、 Biology 、 Reactive oxygen species 、 Mycotoxin 、 Biochemistry 、 Viability assay
摘要: Porcine granulosa cells were cultured in the absence or presence of 10% porcine follicular fluid (FF) at different concentrations (0-20 μM) mycotoxins alternariol (AOH) and beauvericin (BEA). The analyses performed after exposure to these a medium supplemented not with FF harvested from gilts sows. Cell enzymatic activity nuclear membrane integrity evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide ethidium homodimer-1 labelling. Trolox equivalent antioxidant capacity was measured calculate counteract reactive oxygen species. qRT-PCR used determine relative gene expression efflux transporters (ABCG1 ABCG2) as well CYP11 CYP19. Mycotoxin cytotoxicity more related than damage no direct relationship oxidative stress observed, except when exposed AOH. In this case, supplementation sows increased t...
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