Infrequent alterations of the p15, p16, CDK4 and cyclin D1 genes in non-astrocytic human brain tumors.
作者： Kazufumi Sato , Barbara Schäuble , Paul Kleihues , Hiroko Ohgaki , None
DOI: 10.1002/(SICI)1097-0215(19960503)66:3<305::AID-IJC6>3.0.CO;2-2
关键词: Glioma 、 Exon 、 Cancer research 、 Gene 、 Biology 、 Southern blot 、 Cyclin D1 、 Neuroectodermal tumor 、 Tumor suppressor gene 、 Oligodendroglioma
摘要: While several genetic alterations associated with the evolution of astrocytomas have been identified, molecular basis non-astrocytic brain tumors has remained largely unknown. In this study, p15, p16, CDK4 and cyclin D1 genes were analyzed in 69 nonastrocytic human tumors, including 17 oligodendrogliomas, 16 medulloblastomas/primitive neuroectodermal (PNETs), 14 ependymomas 22 meningiomas. Southern blot analysis DNA from frozen samples showed no homozygous deletions p15 or p16 any these tumors. No mobility shift was found by PCR-single-strand conformation polymorphism (PCR-SSCP) exons 1 2 gene genes, except for one oligodendroglioma. Direct sequencing tumor a G A transition at nucleotide 436 (codon 140) exon gene, which is common polymorphism. analyses revealed amplification neoplasms analyzed. contrast to astrocytic show frequent loss alteration appears be rare other nervous system. © 1996 Wiley-Liss, Inc.
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