Characterisation of early and late bovine papillomavirus protein expression in equine sarcoids

摘要: Sarcoids are common skin tumours of horses and donkeys that characterised by persistent proliferation dermal fibroblasts associated with the presence bovine papillomavirus (BPV) DNA. Some early BPV proteins have been demonstrated within sarcoids RNA containing both late transcripts is present, yet it remains unclear whether replication BPV, culminating in production infectious virus particles, can occur equids. Here we report BPV1 isolated from equine encodes a unique deletion four residues L2 protein suggesting novel variant has evolved equines. Such viral evolution would require transmission particles among sarcoids. Quantitative RT-PCR mRNA gene message sarcoid tissues BPV-E2 antigen was detected immunofluorescence nuclei fibroblasts, but no E2 expression could be overlying epidermis where productive expected to occur. Although immunohistochemistry clearly cells samples papillomas, formalin-fixed tissue sarcoids; either dermis or epidermis. Moreover, quantitative represented <0.3% transcribed RNA. We conclude does not undergo infection at levels comparable occurring its natural host.