17β-estradiol differently affects osteogenic differentiation of mesenchymal stem/stromal cells from adipose tissue and bone marrow.

摘要: Adipose-derived and bone marrow stem/stromal cells (ASCs BMSCs) have been often compared for their application in regenerative medicine, several factors sustaining differentiation efficacy investigated. 17 β-estradiol (E2) has reported to influence some functions of progenitor cells. Here we studied the effects 10 100nM E2 on ASC BMSC vitality, proliferation towards osteogenic adipogenic lineages. did not modulate vitality growth rate, while hormone produced a pro-adipogenic effect both mesenchymal (MSCs). In particular, synergy between 7-day pre-treatment led most evident result, increasing lipid vacuoles formation ASCs BMSCs +44% +82%, respectively. Despite fact that alter collagen deposition osteo-induced MSCs, observed different modulation alkaline phosphatase (ALP) activity. Indeed, this marker was always enhanced by BMSCs, with increased it about 70%. contrast, weakened potential, reducing ALP activity 20%, isolated from pre-menopausal women (-30%). Finally, identified an estrogen receptor α (ERα) variant 37kDa expressed MSCs. Interestingly, stimuli drastically reduced its expression, ones mildly isoform only. conclusion, positively affected process MSCs favored induction only, progenitors novel ER-α whose expression modulated during differentiation.