A liver-stage-specific antigen of Plasmodium falciparum characterized by gene cloning.
作者： Claudine Guerin-Marchand , Pierre Druilhe , Bénédicte Galey , Arturo Londono , Jintana Patarapotikul
DOI: 10.1038/329164A0
关键词: Blood serum 、 Malaria 、 Plasmodium falciparum 、 Molecular cloning 、 Antigen 、 Gene 、 Antibody 、 Virology 、 Biology 、 Monoclonal antibody
摘要: The liver phase of development malaria parasites has been studied only recently and remains poorly understood compared to the other stages such as sporozoites, merozoites gametes1,2. Access forms Plasmodium falciparum improved by in vivo3 vitro4 propagation methods, but yield mature schizonts limited does not allow a detailed antigenic analysis. To date, immunofluores-cence assays (IFA) have permitted description species liver-stage-specific antigen(s) (LSA; ref. 3). Monospecific antibodies these antigens obtained due either difficulty immunizing mice (against LSA), or poor stability human monoclonal antibodies. Therefore, means characterizing LSA, we used an alternative immunological approach identify clones corresponding LSA genes. We describe here isolation DNA sequence coding for P. antigen composed repeats 17 amino-acids, which is immunogenic man.
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