cDNA arrays and immunohistochemistry identification of CD10/CALLA expression in hepatocellular carcinoma.

摘要: The histological diagnosis of hepatocellular carcinoma (HCC) can be complicated by difficulty in differentiation from cholangiocarcinoma and metastatic carcinoma. Immunohistochemical stains currently use are suboptimal terms specificity sensitivity. Using cDNA array analysis for differential gene expression, we demonstrated a significant increase mRNA expression level CD10/CALLA, type 2 cell-surface metalloproteinase, HCC, which was subsequently confirmed reverse transcriptase-polymerase chain reaction Western blotting analysis. To test the possibility using CD10/CALLA as diagnostic marker various intrahepatic tumors were studied immunohistochemically monoclonal antibody CD10. A characteristic canalicular-staining pattern observed normal hepatocytes at apical surface bile duct epithelial cells. canalicular CD10 identified 9 15 HCCs examined (60%), whereas 10 cholangiocarcinomas 8 carcinomas lacked this staining. In three six negative CD10, surrounding nonneoplastic liver tissue also negative, suggesting fixation-associated loss immunoreactivity. Six had stronger staining tumor cells when compared to tissue. Three cases benign adenomas expressed luminal aspect. One MCs showed diffuse, cytoplasmic readily distinguishable that HCC. panel other immunohistochemical markers comparison, including polyclonal anti-carcinoembryonic antigen, cytokeratin (CK) 7, CK20, α-fetoprotein. Our results demonstrate arrays effectively used identify new markers, is reliable identifying particularly conjunction with (polyclonal CK7, α-fetoprotein) distinction cholangiocarcinoma.