Association of increased phosphatidylinositol 3-kinase signaling with increased invasiveness and gelatinase activity in malignant gliomas.
作者： Tomas Kubiatowski , Taichang Jang , Mahesh B. Lachyankar , Rebecca Salmonsen , Roya R. Nabi
DOI: 10.3171/JNS.2001.95.3.0480
关键词: Wortmannin 、 Tumor progression 、 Tensin 、 Phosphatidylinositol 、 Cancer research 、 Lipid signaling 、 PTEN 、 Gelatinase 、 Phosphatidylinositol 3-kinase signaling 、 Biology
摘要: Object. Glioblastoma multiforme is the most malignant of primary brain tumors and aggressively infiltrates surrounding tissue, resulting in distant foci within central nervous system, thereby rendering this tumor surgically incurable. The recent findings that both phosphatidylinositol 3-kinase (PI 3-K) phosphatase tensin homolog (PTEN) regulate cell invasiveness have led authors to surmise these lipid signaling molecules might play a role regulating matrix metalloproteinases (MMPs), which are essential for invasion. Methods. Using C6 glioma line, does not express measurable amounts PTEN protein vitro MMP dependent, determined was significantly reduced when cells were preincubated overnight with LY294002 or wortmannin, two specific inhibitors PI 3-K signaling. Next, using gelatin zymography, it noted compounds inhibited M...
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