Multiple breakpoints within the BCL-1 locus in B-cell lymphoma: rearrangements of the cyclin D1 gene

摘要: Abstract Centrocytic lymphoma (CC) and intermediately differentiated lymphocytic (IDL) are B-cell non-Hodgkin9s lymphomas composed of lymphocytes presumably derived from follicle mantle cells. In these lymphomas, a specific chromosomal translocation, t(11;14)(q13;q32), has been described. Previous studies suggested an association between t(11;14) translocations BCL-1 rearrangements. To evaluate the rearrangements CC/IDL, Southern blot analysis was performed on panel 20 cases 22 morphologically similar 11 chronic leukemias, 2 myelomas. We used various probes covering considerable proportion 120-kilobase locus, in 50% CC/IDL (10 20) were detected. CC, all 4 breakpoints located at major translocation cluster (MTC). contrast, IDL, detected 3 different regions: MTC, with breakpoint 24 kilobases outside additional found 5′ first exon PRAD1/CCND1 gene, which is 120 MTC. addition, one leukemia showed 63 cases, there comigration rearranged 11q13 fragment immunoglobulin heavy chain-joining gene complex, indicating t(11;14)(q13;q32) rearrangement. Our results show that helpful to identify but multiple present over large region, therefore, many necessary cover breakpoints.