Detection of K-ras mutations in lung carcinomas: relationship to prognosis.

摘要: The K-ras mutation is one of the most common genetic alterations found in human lung cancer. To evaluate prognostic value ras gene cancer a U.S. population, we have screened 173 tumors, which included 127 adenocarcinomas, 37 squamous carcinomas, and 9 adenosquamous for mutations using combination PCR denaturing gradient gel electrophoresis. Forty-three tumors contained mutations. Of these, 41 were identified among adenocarcinomas (32%), 1 carcinomas (2.7%), (11%). Forty these codon 12 consisted 24 G to T transversions, A transitions, 2 C double GG TT mutation. Two other transversions 13, transversion was 61. data showed that gender did not seem affect incidence types or amino acid changes. Examination relation overall survival no difference with compared K-ras-negative adenocarcinomas. However, substitution wild-type GGT (glycine) at GTT (valine) CGT (arginine) strong trend (P = 0.07) toward poorer prognosis substitutions. Substitution glycine aspartate (GAT) 0.06) better outcome than valine arginine substitution. Although trends will require larger patient populations verification, suggest significance may depend on p21(ras) protein.