Biological properties of human c-Ha-ras1 genes mutated at codon 12
作者： Peter H. Seeburg , Wendy W. Colby , Daniel J. Capon , David V. Goeddel , Arthur D. Levinson
DOI: 10.1038/312071A0
关键词: Mutation 、 Mutant 、 Gene 、 Point mutation 、 Mutagenesis (molecular biology technique) 、 Peptide sequence 、 Amino acid 、 Molecular biology 、 Biology 、 Codon usage bias
摘要: Vertebrate genomes contain proto-oncogenes whose enhanced expression or alteration by mutation seems to be involved in the development of naturally occurring tumours. These activated genes, usually assayed their ability induce malignant transformation NIH 3T3 cells, are frequently related ras oncogene Harvey (Ha-ras) Kirsten (Ki-ras) murine sarcoma viruses, a third member this family (N-ras). Activation involves point which often affect codon 12 (refs 16-26) encoded 21,000-molecular weight polypeptide (p21). To provide insight into structural requirements p21 activation, we have now constructed 20 mutant c-Ha-ras1 genes vitro mutagenesis, each encoding different amino acid at 12. Analysis rat fibroblasts transfected with these altered demonstrates that all acids except glycine (which is normal cellular genes) and proline position activate p21, suggesting requirement for an alpha-helical structure region polypeptide. The morphological phenotype cells transformed can, however, depend on particular position.
nature.com
core.ac.uk
mpg.de
harvard.edu
sci-hub.se 参考文章(51)
E Santos, D Martin-Zanca, E. Reddy, M. Pierotti, G Della Porta, M Barbacid, Malignant activation of a K-ras oncogene in lung carcinoma but not in normal tissue of the same patient Science. ,vol. 223, pp. 661- 664 ,(1984) , 10.1126/SCIENCE.6695174
W. Benton, R. Davis, Screening lambdagt recombinant clones by hybridization to single plaques in situ Science. ,vol. 196, pp. 180- 182 ,(1977) , 10.1126/SCIENCE.322279
Melissa S. McCoy, John J. Toole, James M. Cunningham, Esther H. Chang, Douglas R. Lowy, Robert A. Weinberg, Characterization of a human colon/lung carcinoma oncogene Nature. ,vol. 302, pp. 79- 81 ,(1983) , 10.1038/302079A0
E. Premkumar Reddy, Roberta K. Reynolds, Eugenio Santos, Mariano Barbacid, A point mutation is responsible for the acquisition of transforming properties by the T24 human bladder carcinoma oncogene Nature. ,vol. 300, pp. 149- 152 ,(1982) , 10.1038/300149A0
Daniel J. Capon, Ellson Y. Chen, Arthur D. Levinson, Peter H. Seeburg, David V. Goeddel, Complete nucleotide sequences of the T24 human bladder carcinoma oncogene and its normal homologue Nature. ,vol. 302, pp. 33- 37 ,(1983) , 10.1038/302033A0
L. Feig, R. Bast, R. Knapp, G. Cooper, Somatic activation of rasK gene in a human ovarian carcinoma Science. ,vol. 223, pp. 698- 701 ,(1984) , 10.1126/SCIENCE.6695178
A. Eva, S. R. Tronick, R. A. Gol, J. H. Pierce, S. A. Aaronson, Transforming genes of human hematopoietic tumors: frequent detection of ras-related oncogenes whose activation appears to be independent of tumor phenotype Proceedings of the National Academy of Sciences of the United States of America. ,vol. 80, pp. 4926- 4930 ,(1983) , 10.1073/PNAS.80.16.4926
H Land, L. Parada, R. Weinberg, Cellular oncogenes and multistep carcinogenesis. Science. ,vol. 222, pp. 771- 778 ,(1983) , 10.1126/SCIENCE.6356358
K. Shimizu, M. Goldfarb, Y. Suard, M. Perucho, Y. Li, T. Kamata, J. Feramisco, E. Stavnezer, J. Fogh, M. H. Wigler, Three human transforming genes are related to the viral ras oncogenes Proceedings of the National Academy of Sciences of the United States of America. ,vol. 80, pp. 2112- 2116 ,(1983) , 10.1073/PNAS.80.8.2112
Clifford J. Tabin, Scott M. Bradley, Cornelia I. Bargmann, Robert A. Weinberg, Alex G. Papageorge, Edward M. Scolnick, Ravi Dhar, Douglas R. Lowy, Esther H. Chang, Mechanism of activation of a human oncogene. Nature. ,vol. 300, pp. 143- 149 ,(1982) , 10.1038/300143A0