Hypoxia/reoxygenation stress increases markers of vaso-occlusive crisis in sickle SAD mice.

摘要: In sickle cell disease, the factors involved in vasoocclusive crisis (VOC) include sickling of red blood cells (RBC), abnormal rheology, inflammation, vascular adhesion, oxidative stress, coagulation, and tone modulation. The aim this study was to further characterize molecular response some VOC by inducing a hypoxia/reoxygenation stress SAD mice. Results show that mice can induce: (i) decrease reticulocytes count, mean corpuscular volume along with an increase lactate dehydrogenase (p = 0.07) sickled proportion; (ii) significant lung VCAM-1, ICAM-1, IL-1β, ET-1, eNOS, TF mRNA associated VCAM-1 expression on endothelium; (iii) rise cardiac increased lipid oxidation decreased anti-oxidant enzyme activities, (iv) plasma TNF-α IL-6 ET-1. mice, induces hemolysis that, together may induce occlusion consequently RBC sickling. present results give kinetics markers which aid testing efficiency new therapeutic processes against VOC.