Online in-tube solid-phase extraction using a nonfunctionalized PVC tube coupled with ICPMS for in vivo monitoring of trace metals in rat brain microdialysates.

摘要: We have developed a simple, automatic, online in-tube solid-phase extraction (SPE) method for the preconcentration of trace elements from saline samples. The takes advantage adsorption metal ions onto interior nonfunctionalized PVC tube as means separation matrix salt. transition is presumably dominated by Lewis acid/base interactions, which facilitate formation metal-PVC complexes. For simultaneous determination multiple metals in extracellular fluid brains anesthetized rats, we an analytical system comprising microdialysis sampling, established procedure, and ICPMS. In step, efficiency was optimal when pH sample adjusted to 8.0 using phosphate buffer. After tube, adsorbed analytes were eluted with 0.5% HNO 3 prior ICPMS measurement. those present at less than nanogram-per-milliliter concentrations (i.e., Cu, Zn, Mn), temporal resolution 12 min required collect enough microdialysate meet sensitivity requirements instrument. Noteworthily, because washing postconditioning are not necessary, relatively unsophisticated clean procedure attained extremely low detection limits range 5.0 510 ng L (-1) thus obtained analysis Mn, Pb, Cd, Ni, Co samples 8 microL To best our knowledge, this study first exploit peristaltic tubing SPE adsorbent laboratory-on-valve mode treatment confirmed reliability through certified reference material SLEW-3 (estuarine water) 2670a (human urine) demonstrated its applicability vivo monitoring basal concentration profiles brain living rat.