Isolation and characterization of the promoter of the human prostate cancer-specific DD3 gene.

摘要: Recently, we have described a novel gene,DD3, which is one of the most prostate cancer-specific genes to date (Bussemakers, M. J. G., van Bokhoven, A., Verhaegh, G. W., Smit, F. P., Karthaus, H. M., Schalken, Debruyne, J., Ru, N., and Isaacs, W. B. (1999) Cancer Res. 59, 5975–5979). The expression DD3 indicates that theDD3 gene promoter promising tool for treatment cancer. To identify elements are responsible DD3, isolated characterized promoter. Sequence analysis 5′-flanking region was performed several promoter-human growth hormone reporter constructs were prepared, transiently transfected in DD3-positive cell line LNCaP DD3-negative lines. Using 500-base pair construct, could detect activity cells, not affected by increasing size constructs. Truncated constructs, however, showed an increased transcriptional activity, suggesting presence silencer negatively regulates DD3. DNase-I footprint analysis, using nuclear extracts from revealed three DNase-I-protected areas within proximal We show high mobility group I(Y) protein binds recruits another, yet unidentified, cells.