Novel Regulation of Type IV Collagenase (Matrix Metalloproteinase-9 and -2) Activities by Transforming Growth Factor-β1 in Human Prostate Cancer Cell Lines

作者: Inder Sehgal , Timothy C. Thompson

DOI: 10.1091/MBC.10.2.407

关键词: CollagenaseSecretionTransforming growth factor betaGelatinasesMetalloproteinaseCell biologyTransforming growth factorMolecular biologyCell typeBiologyGene expression

摘要: The type IV collagenases/gelatinases matrix metalloproteinase-2 (MMP-2) and MMP-9 play a variety of important roles in both physiological pathological processes are regulated by various growth factors, including transforming factor-β1 (TGF-β1), several cell types. Previous studies have suggested that cellular control one or collagenases can occur through direct transcriptional mechanisms and/or after secretion proenzyme processing interactions with metalloproteinase inhibitors. Using human prostate cancer lines, we found TGF-β1 induces the proenzyme; however, this induction does not result from effects on gene transcription but, instead, protein synthesis–requiring process leading to increased mRNA stability. In addition, examined levels regulation MMP-2 line higher secreted collagenase stability 72-kDa proenzyme. These results identify two novel nontranscriptional pathways for expression activities.

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