Ectopic Neo-Formed Intracellular Membranes in Escherichia coli: A Response to Membrane Protein-Induced Stress Involving Membrane Curvature and Domains

摘要: Bacterial cytoplasmic membrane stress induced by the overexpression of proteins at high levels can lead to formation ectopic intracellular membranes. In this review, we report various observations such membranes in Escherichia coli, compare their morphological and biochemical characterizations, analyze underlying molecular processes leading formation. Actually, these display either vesicular or tubular structures, are separated connected membrane, present mono- polydispersed sizes shapes, possess ordered disordered arrangements. Moreover, composition differs from that with amounts overexpressed protein altered lipid-to-protein ratio cardiolipin content. These data reveal importance domains, based on local specific lipid–protein protein–protein interactions, both being crucial for curvature generation, they highlight strong influence structure. Indeed, whether cylindrically spherically curvature-active actively curvogenic passively curvophilic, scenarios different be correlated features neo-formed internal Delineating mechanisms is highly desirable a better understanding protein–lipid interactions within optimization high-level production E. coli.